Product Name :
HSP70/HSP72 polyclonal antibody
Sequence:
Purity:
Molecular Weight:
Solubility :
Appearance:
Use/Stability :
Description:
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.{{1691249-45-2} MedChemExpress|{1691249-45-2} Biological Activity|{1691249-45-2} References|{1691249-45-2} supplier} Western blot analysis of HSP70 pAb: Lane 1: HSP70 (HSP72) Recombinant Human Protein, Lane 2: HSC70 (HSP73) Recombinant Bovine Protein (negative control), Lane 3: HeLa Cell Lysate, Heat Shocked, Lane 4: PC-12 Cell Lysate, Heat Shocked, Lane 5: Vero Cell Lysate, Heat Shocked, Lane 6: CHO-K1 Cell Lysate, Heat Shocked Western blot analysis of HSP70 pAb: Lane 1: HSP70 (HSP72) Recombinant Human Protein, Lane 2: HSC70 (HSP73) Recombinant Bovine Protein (negative control), Lane 3: HeLa Cell Lysate, Heat Shocked, Lane 4: PC-12 Cell Lysate, Heat Shocked, Lane 5: Vero Cell Lysate, Heat Shocked, Lane 6: CHO-K1 Cell Lysate, Heat Shocked
CAS :
Solubility:
Formula:
Additional Information :
| Alternative Name Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72 | Application IP, WB | Application Notes Detects a band of ~70kDa by Western blot.{{2760481-53-4} medchemexpress|{2760481-53-4} Purity & Documentation|{2760481-53-4} References|{2760481-53-4} manufacturer} | Formulation Liquid.PMID:29261870 In PBS containing 50% glycerol and 0.09% sodium azide. | GenBank ID M11717 | Gene/Protein Identifier NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM) | Host Rabbit | Immunogen Synthetic peptide corresponding to a portion of human Hsp70. | Recommendation Dilutions/Conditions Western Blot (1:1,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Source Purified from rabbit serum. | Species Reactivity Beluga, Bovine, Dog, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep | UniProt ID P0DMV8 (HSPA1A), P0DMV9 (HSPA1B) | Unit of Measure (UM) µg