Name :
NCR3 (Human) Recombinant Protein
Biological Activity :
Human NCR3 (AAH52582, Leu19-Thr138) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro
Tag :
Result of bioactivity analysis
Protein Accession No. :
AAH52582
Protein Accession No.URL :
https://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=259197
Amino Acid Sequence :
Leu19-Thr138
Molecular Weight :
39.8
Storage and Stability :
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.Aliquot to avoid repeated freezing and thawing.
Host :
Human
Interspecies Antigen Sequence :
Preparation Method :
Mammalian cell (HEK293) expression system
Purification :
Quality Control Testing :
SEC-HPLC and Tris-Bis PAGE SEC-HPLC The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC. Tris-Bis PAGE Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Storage Buffer :
Lyophilized from sterile distilled Water is > 100 ug/mL
Applications :
Enzyme-linked Immunoabsorbent Assay， Immobilized Human NKp30, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 13.4 ng/mL determined by ELISA (QC Test). Functional Study， SDS-PAGE， Surface Plasmon Resonance， Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 23.10 nM as determined in SPR assay (Biacore T200).
Gene Name :
NCR3
Gene Alias :
1C7, CD337, LY117, MALS, NKp30
Gene Description :
natural cytotoxicity triggering receptor 3
Gene Summary :
Natural cytotoxicity receptors (NCRs), such as NCR3, are activating natural killer (NK) cell receptors that belong to the immunoglobulin (Ig) superfamily. NCR3 is expressed in all resting and activated NK cells and forms a complex with CD3-zeta (CD3Z, or CD247; MIM 186780) (Sato et al., 2001 [PubMed 11782277]).[supplied by OMIM
Other Designations :
OTTHUMP00000029162|OTTHUMP00000038859|OTTHUMP00000038863|lymphocyte antigen 117
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