HIV-1 TAT Protein

Description:
|Introduction Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication (Jones KA, 1994) and infectivity (Arya SK, 1985; Fisher AG, 1986). In addition, during acute infection, Tat is released extracellularly by infected cells (Chang HC, 1997; Ensoli B, 1990) and is taken up by neighboring cells where it transactivates viral replication (Ensoli B, 1993) and increases virus infectivity. HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in |E. coli-derived. Predicted molecular mass: 14.4 kDa. Purity > 90%, by SDS-|Lyophilized with 0.1% glycerol. Reconstitution: It is recommended to reconstitute the lyophilized HIV-1 TAT Cys 22 in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Applications: Recognized by anti-Tat (HIV-1) polyclonal antibody. Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum. Shipping: The product is shipped at a|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |Other HIV-1 TAT Proteins: Recombinant HIV-1 TAT, Biotin Labeled Recombinant HIV-1 TAT Clade-D Recombinant HIV-1 TAT Clade-C Recombinant HIV-1 TAT Clade-B Recombinant HIV-1 TAT Clade-A