Name :
Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein (His Tag)
Biological Activity :
Background :
Biological Activity :
1.Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR4 (Cat:50036-M08H) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:10702-HNAC). The EC50 of human IgG1 (Fc) (Cat:10702-HNAC) is 0.11-0.25 μg/ml.2.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag (Cat. No. 50036-M08H) with an affinity constant of 3.43nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).3.Loaded Recombinant Mouse CD32B/Fcgr2b Protein, His Tag (Cat. No. 50030-M08H) on His1K Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 36.2nM as determined in BLI assay (Sartorius Octet RED384)(Routinely tested).
Expression Host :
Mouse
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_653142.2
NCBI Gene ID :
Synonyms :
Synonyms :
Fc receptor, IgG, low affinity IV
Amino Acid Sequence :
Molecular Weight :
The recombinant mouse FCGR4 consists of 194 amino acids and has a predicted molecular mass of 22.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
The amino acid sequence corronding to Met 1-Gln 203 of mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
4833442P21Rik Protein, Mouse; CD16-2 Protein, Mouse; FcgammaRIV Protein, Mouse; Fcgr3a Protein, Mouse; FcgRIV Protein, Mouse; Fcrl3 Protein, Mouse CD16-2/FCGR4/Fc gamma RIV 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
CDCP1 Antibody MedChemExpress Oleic acid custom synthesis PMID:35119557 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com