This study introduces a highly efficient, rapid, and selective colorimetric sensor for the detection of bisphenol A (BPA) using a newly synthesized chromophore, 3′,6′-bis(diethylamino)-2-((3,4,5-trimethylbenzylidene)amino)spiro[isoindoline-1,9′-xanthen]-3-one (BTSIXO), conjugated with Fe³⁺ ions. The sensor was developed through a simple, eco-friendly one-pot synthesis protocol that takes only 20 minutes and yields high-purity product with excellent reproducibility. The BTSIXO-Fe³⁺ conjugate exhibits strong absorbance at 306 nm and 343 nm in DMSO solvent, and upon interaction with Fe³⁺ ions, undergoes a distinct color shift from colorless to dark pink, confirming successful complex formation. Upon exposure to BPA, the conjugate displays a progressive reduction in absorbance intensity, accompanied by a visible color change from magenta to violet, enabling both qualitative and quantitative analysis. The sensor demonstrates an outstanding linear range from 0.1 to 150 ppm with a limit of detection as low as 0.02 ppm, outperforming many existing methods in sensitivity and dynamic range. Selectivity studies confirmed minimal interference from common biomolecules such as glucose, dopamine, creatinine, urea, uric acid, and even structurally similar estrogen, due to the specific coordination chemistry between BPA’s phenolic hydroxyl groups and Fe³⁺ within the BTSIXO cavity. Competitive assays further validated the sensor’s robustness under complex matrices. The platform was successfully applied to quantify BPA in various real samples: drinking water stored in plastic bottles from five different commercial brands, and fish organs (liver and ovary) from Oreochromis mossambicus fingerlings exposed to BPA-spiked laboratory water or collected from industrial effluents including plastic, textile, dye, and tannery sources. Histopathological evaluations revealed severe hepatic necrosis and early ovarian maturation in exposed fish, correlating with elevated BPA levels.mTOR Antibody Epigenetics Quantitative results obtained via UV-Vis spectrometry were validated against ESI-MS data, showing excellent agreement and confirming the sensor’s accuracy.CK7 Antibody supplier The entire analytical procedure, including sample preparation and measurement, is completed within five minutes, making it ideal for on-site monitoring and routine screening.PMID:34823271 This work presents a powerful, cost-effective, and user-friendly tool for environmental and biological monitoring of BPA contamination, offering a significant advancement in field-deployable sensing technology.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com