Anti-TLR4 Antibody

Anti-TLR4 Antibody__Rabbit Anti-Human TLR4 Polyclonal Danoprevir

Product Name	TLR4 Antibody
Description	Rabbit Anti-Human TLR4 Polyclonal
Species Reactivity	Human, Mouse
Applications	, WB , IHC , ICC/IF , FCM , FACS
Antibody Dilution	WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.
Host Species	Rabbit
Immunogen Species	Human
Immunogen	Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Concentration	1 mg/ml
Conjugates	Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated APC (Allophycocyanin) Overview: High quantum yield Large phycobiliprotein 6 chromophores per molecule Isolated from red algae Molecular Weight: 105 kDa APC Datasheet   Optical Properties: λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5     ATTO 390 Overview: High fluorescence yield Large Stokes-shift (89 nm) Good photostability Moderately hydrophilic Good solubility in polar solvents Coumarin derivate, uncharged Low molar mass: 343.42 g/mol  ATTO 390 Datasheet Optical Properties: λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm     ATTO 488 Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation New dye with net charge of -1 Molar Mass: 804 g/mol  ATTO 488 Datasheet    Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC    ATTO 565 Overview: High fluorescence yield High thermal and photostability Good solubility in polar solvents Excellent solubility in water Very little aggregation Rhodamine dye derivative Molar Mass: 611 g/mol ATTO 565 Datasheet   Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC    ATTO 594 Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation New dye with net charge of -1 Molar Mass: 1137 g/mol ATTO 594 Datasheet   Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red®    ATTO 633 Overview: High fluorescence yield High thermal and photostability Moderately hydrophilic Good solubility in polar solvents Stable at pH 4 – 11 Cationic dye, perchlorate salt Molar Mass: 652.2 g/mol ATTO 633 Datasheet Optical Properties: λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5    ATTO 655 Overview: High fluorescence yield High thermal and photostability Excellent ozone resistance Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 634 g/mol ATTO 655 Datasheet Optical Properties: λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5    ATTO 680 Overview: High fluorescence yield Excellent thermal and photostability Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 631 g/mol ATTO 680 Datasheet   Optical Properties: λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5    ATTO 700 Overview: High fluorescence yield Excellent thermal and photostability Quenched by electron donors Very hydrophilic Good solubility in polar solvents Zwitterionic dye Molar Mass: 575 g/mol ATTO 700 Datasheet   Optical Properties: λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5     FITC (Fluorescein) Overview: Excellent fluorescence quantum yield High rate of photobleaching Good solubility in water Broad emission spectrum pH dependent spectra Molecular formula: C20H12O5 Molar mass: 332.3 g/mol FITC-Fluorescent-conjugate Optical Properties: λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC    PE/ATTO 594 PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. Overview: High fluorescence yield High photostability Very hydrophilic Excellent solubility in water Very little aggregation PE/ATTO 594 Datasheet Optical Properties: λex = 535 nm λem = 627 nm Laser = 488 to 561 nm    PerCP  Overview: Peridinin-Chlorophyll-Protein Complex Small phycobiliprotein Isolated from red algae Large stokes shift (195 nm) Molecular Weight: 35 kDa PerCP Datasheet   Optical Properties: λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm     R-PE (R-Phycoerythrin) Overview: Broad excitation spectrum High quantum yield Photostable Member of the phycobiliprotein family Isolated from red algae Excellent solubility in water Molecular Weight: 250 kDa R-PE Datasheet   Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC   AP (Alkaline Phosphatase) Properties: Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation Catalyzes the conversion of: Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products Molecular weight: 140 kDa Applications: Western blot, immunohistochemistry, and ELISA AP Datasheet HRP (Horseradish peroxidase) Properties: Enzymatic activity is used to amplify weak signals and increase visibility of a target Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors Catalyzes the conversion of: Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL) Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products High turnover rate enables rapid generation of a strong signal 44 kDa glycoprotein Extinction coefficient: 100 (403 nm) Applications: Western blot, immunohistochemistry, and ELISA HRP Datasheet Biotin Properties: Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity Molar mass: 244.31 g/mol Formula: C10H16N2O3S Applications: Western blot, immunohistochemistry, and ELISA Biotin Datasheet Streptavidin Properties: Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity Molecular weight: 53 kDa Formula: C10H16N2O3S Applications: Western blot, immunohistochemistry, and ELISA Streptavidin Datasheet

Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein A purified
Clonality	Polyclonal
Specificity	Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Cite This Product	Rabbit Anti-Human TLR4 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-200)
Certificate of Analysis	2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Alternative Names	ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody
Research Areas	Atherosclerosis, Cardiovascular System, Cell Signaling
Cellular Localization	Membrane
Accession Number	NP_612564.1
Gene ID	7099
Swiss Prot	O00206
Scientific Background	The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
References	1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.