Anti-IGF-1 Antibody__Rabbit Anti-Human IGF-1 Polyclonal Y-27633 (dihydrochloride)
Anti-IFT88 Antibody
Anti-IFT88 Antibody__Rabbit Anti-Human IFT88 Polyclonal SLx-2120
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects ~94 kDa.
Cite This Product
Rabbit Anti-Human IFT88 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-679)
Certificate of Analysis
A 1:1000 dilution of SPC-679 was sufficient for detection of IFT88 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132915
Alternative Names
D13S1056E Antibody, TPR repeat protein 10 Antibody, Tetratricopeptide repeat domain 10 Antibody, Ift88 Antibody, Recessive polycystic kidney disease protein Tg737 homolog Antibody, IFT88_HUMAN Antibody, Polaris homolog Antibody, Probe hTg737 (polycystic kidney disease, autosomal recessive) Antibody, TG737 Antibody, TTC10 Antibody, DAF19 Antibody, IFT88 Antibody, hTg737 Antibody, Intraflagellar transport 88 homolog Antibody, MGC26259 Antibody, Tetratricopeptide repeat protein 10 Antibody, Intraflagellar transport protein 88 homolog Antibody, RP11-172H24.2 Antibody
Cellular Localization
Cytoplasm, Centriole, Centrosome, Cilium Basal Body, Cytoskeleton, Microtubule Organizing Center
Accession Number
NP_001305420.1
Gene ID
8100
Swiss Prot
Q13099
Anti-IFN gamma Antibody
Anti-IFN gamma Antibody__Rabbit Anti-Human IFN gamma Polyclonal AV-413
Product Name
IFN gamma Antibody
Description
Rabbit Anti-Human IFN gamma Polyclonal
Species Reactivity
Human
Applications
,
WB
,
IHC
Antibody Dilution
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A synthesized peptide derived from human Interferon gamma
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet
Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:
Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:
Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:
Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:
Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:
Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:
Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:
Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:
Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:
Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:
Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS, pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Affinity Purified
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects ~19kDa, endogenous levels of total Interferon gamma
Cite This Product
Rabbit Anti-Human IFN gamma Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-1297)
Certificate of Analysis
A 1:1000 dilution of SPC-1297 was sufficient for detection of IFN gamma in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132789
Alternative Names
IFG Antibody, IFI Antibody, IFN gamma Antibody, IFN, immune Antibody, IFN-gamma Antibody, IFNG Antibody, IFNG_HUMAN Antibody, Immune interferon Antibody, Interferon gamma Antibody
Research Areas
Cancer
Cellular Localization
Secreted
Accession Number
NP_000610.2
Gene ID
3458
Swiss Prot
P01579
Scientific Background
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. It is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-gamma production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-gamma is inducible by IL-2, FGF and EGF. The active form of IFN-gamma is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-gamma is stabilized by non-covalent interactions through the interface of the helices. IFN-gamma tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence. 20 kDa and 25 kDa forms exist due to variable glycosylation; 40-60 kDa forms are observable under non-denaturing conditions as dimers and tetramers.
References
1. Gray PW et al. (1982) Nature 298 (5877): 859–63.
2. Wheelock EF et al. (1965) Science 149, 310-311, 1965.
3. Schoenborn J.R. and Wilson C.B. (2007) Adv. Immunol. 96: 41–101.
Rabbit Anti-Human IFN gamma Polyclonal
WB
,
IHC
| APC (Allophycocyanin) | ||
Overview:
|
|
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
| ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
|
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| ATTO 488 | ||
| Overview: | |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 565 | ||
| Overview: | |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 594 | ||
| Overview: | |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 633 | ||
| Overview: | |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 655 | ||
| Overview: | |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 680 | ||
| Overview: | |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 700 | ||
Overview:
|
|
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| FITC (Fluorescein) | ||
| Overview: | |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| PE/ATTO 594 | ||
| PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
| Overview: | |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| PerCP | ||
| Overview: | |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| R-PE (R-Phycoerythrin) | ||
| Overview: | |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
2. Wheelock EF et al. (1965) Science 149, 310-311, 1965.
3. Schoenborn J.R. and Wilson C.B. (2007) Adv. Immunol. 96: 41–101.
Idebenone
Idebenone__Quinone antioxidant BLZ946
Product Name
Idebenone
Description
Quinone antioxidant
Purity
>98% (TLC and HPLC); NMR (Conforms)
CAS No.
58186-27-9
Molecular Formula
C19H30O5
Molecular Weight
338.44
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Antioxidant
Solubility
Soluble to 100 mM in DMSO, 100 mM in ethanol
Source
Synthetic
Appearance
Orange Solid
SMILES
CC1=C(C(=O)C(=C(C1=O)OC)OC)CCCCCCCCCCO
InChI
InChI=1S/C19H30O5/c1-14-15(12-10-8-6-4-5-7-9-11-13-20)17(22)19(24-3)18(23-2)16(14)21/h20H,4-13H2,1-3H3
InChIKey
JGPMMRGNQUBGND-UHFFFAOYSA-N
Safety Phrases
Classification: Caution: Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product
Idebenone (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-150)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132784
Alternative Names
2-(10-Hydroxydecyl)-5,6-dimethoxy-3-methyl-1,4-benzoquinone
Research Areas
Cancer, Oxidative Stress
PubChem ID
3686
Scientific Background
Idebenone has been tested for numerous pharmaceutical indications including Alzheimer’s (1), Friedreich’s ataxia (2), and Duchenne muscular dystrophy. It also has similar antioxidant properties to CoQ10, and has therefore been used in anti-aging applications as well (3).
References
1. Gutzmann H., Kuhl K.P., Hadler D and Rapp M.A. (2002) Pharmacopsychiatry. 35(1): 12-18.
2. Di Prospero N.A., Baker A., Jeffries N, Fischbeck K.H. (2007) Lancet Neuro. 6(10): 878-886.
3. McDaniel D., Neudecker B., Dinardo J, Lewis J., Maibach H. (2005) Cosmet Dermatol. 4(3): 167-173.
Quinone antioxidant
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
2. Di Prospero N.A., Baker A., Jeffries N, Fischbeck K.H. (2007) Lancet Neuro. 6(10): 878-886.
3. McDaniel D., Neudecker B., Dinardo J, Lewis J., Maibach H. (2005) Cosmet Dermatol. 4(3): 167-173.
Anti-ICK Antibody (pTyr156 + pThr157)
Anti-ICK Antibody (pTyr156 + pThr157)__Rabbit Anti-Human ICK (pTyr156 + pThr157) Polyclonal Pacritinib
Product Name
ICK Antibody (pTyr156 + pThr157)
Description
Rabbit Anti-Human ICK (pTyr156 + pThr157) Polyclonal
Species Reactivity
Human
Applications
,
WB
,
AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr156 and Thr157 of human ICK (AA152-158)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet
Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:
Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:
Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:
Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:
Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:
Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:
Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:
Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:
Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:
Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:
Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 71.427 kDa.
Cite This Product
Rabbit Anti-Human ICK (pTyr156 + pThr157) Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-996)
Certificate of Analysis
A 1:250 dilution of SPC-996 was sufficient for detection of ICK (pTyr156 + pThr157) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132736
Alternative Names
ECO Antibody, Heart serine threonine protein kinase Antibody, hICK Antibody, Intestinal cell (MAK like) kinase Antibody, Intestinal cell kinase Antibody, Laryngeal cancer kinase 2 Antibody, LCK2 Antibody, MAK related kinase Antibody, MGC46090 Antibody, mICK Antibody, MRK Antibody, Serine/threonine protein kinase ICK Antibody
Cellular Localization
Cytoplasm, Cytoskeleton, Cytosol, Nucleus
Accession Number
NP_055735
Gene ID
22858
Swiss Prot
Q9UPZ9
Scientific Background
ICK1 encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation.
Rabbit Anti-Human ICK (pTyr156 + pThr157) Polyclonal
WB
,
AM
| APC (Allophycocyanin) | ||
Overview:
|
|
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
| ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
|
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| ATTO 488 | ||
| Overview: | |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 565 | ||
| Overview: | |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 594 | ||
| Overview: | |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 633 | ||
| Overview: | |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 655 | ||
| Overview: | |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 680 | ||
| Overview: | |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 700 | ||
Overview:
|
|
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| FITC (Fluorescein) | ||
| Overview: | |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| PE/ATTO 594 | ||
| PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
| Overview: | |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| PerCP | ||
| Overview: | |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| R-PE (R-Phycoerythrin) | ||
| Overview: | |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
ICG-001
ICG-001__β-catenin inhibitor TEPP-47
Product Name
ICG-001
Description
β-catenin inhibitor
Purity
>98%
CAS No.
847591-62-2
Molecular Formula
C33H32N4O4
Molecular Weight
548.63
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble to 100 mM in DMSO and to 50 mM in ethanol
Source
Synthetic
Appearance
White solid
SMILES
C1CN(email protected2CN(C(=O)C@@H(N2C1=O)CC3=CC=C(C=C3)O)CC4=CC=CC5=CC=CC=C54)C(=O)NCC6=CC=CC=C6
InChI
InChI=1S/C33H32N4O4/c38-27-15-13-23(14-16-27)19-29-32(40)35(21-26-11-6-10-25-9-4-5-12-28(25)26)22-30-36(18-17-31(39)37(29)30)33(41)34-20-24-7-2-1-3-8-24/h1-16,29-30,38H,17-22H2,(H,34,41)/t29-,30+/m0/
InChIKey
HQWTUOLCGKIECB-XZWHSSHBSA-N
Safety Phrases
Classification: Caution. Substance not yet fully tested.
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25 – Avoid contact with skin and eyes
Cite This Product
ICG-001 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-387)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132637
Alternative Names
(6S,9aS)-Hexahydro-6-(4-hydroxyphenyl)methyl-8-(1-naphthalenylmethyl)-4,7-dioxo-N-(phenylmethyl)-2H-pyrazino1,2-apyrimidine-1(6H)-carboxamide
Research Areas
Cell Signaling
PubChem ID
11238147
Scientific Background
Inhibitor of TCF/β-catenin-mediated transcription. Selectively inhibits β-catenin/CBP interaction; displays no effect on β-catenin/p300 interaction. Exhibits growth inhibitory effects in colon carcinoma cell lines (SW480 and HCT-116 cells); also displays efficacy in Min mouse and nude mouse SW620 xenograft models.
References
1. Emami K.H., et al. (2004) Proc. Natl. Acad. Sci. USA. 101(34): 12682-7.
2. De Sousa E.M., et al. (2011) Clin. Cancer Res. 17(4): 647-53.
3. Zhou B., et al. (2012) J. Biol. Chem. 287(10): 7026-38.
β-catenin inhibitor
Safety Phrases:
S22 – Do not breathe dust
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
S24/25 – Avoid contact with skin and eyes
2. De Sousa E.M., et al. (2011) Clin. Cancer Res. 17(4): 647-53.
3. Zhou B., et al. (2012) J. Biol. Chem. 287(10): 7026-38.
Hypericin
Hypericin__Protein kinase C inhibitor Siponimod
Product Name
Hypericin
Description
Protein kinase C inhibitor
Purity
>98%
CAS No.
548-04-9
Molecular Formula
C30H16O8
Molecular Weight
504.45
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble to 10 mM in ethanol and to 50 mM in DMSO
Source
Synthetic
Appearance
Dark Red Solid
SMILES
CC1=CC(=C2C3=C4C5=C(C(=CC(=C5C2=O)O)O)C6=C7C4=C8C(=C13)C(=CC(=C8C(=O)C7=C(C=C6O)O)O)C)O
InChI
InChI=1S/C30H16O8/c1-7-3-9(31)19-23-15(7)16-8(2)4-10(32)20-24(16)28-26-18(12(34)6-14(36)22(26)30(20)38)17-11(33)5-13(35)21(29(19)37)2
InChIKey
BTXNYTINYBABQR-UHFFFAOYSA-N
Safety Phrases
Classification: Harmful. May be harmful if inhaled, swallowed or absorbed through skin.
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Cite This Product
Hypericin (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-250)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132562
Alternative Names
1,3,4,6,8,13-Hexahydroxy-10,11-dimethylphenanthro1,10,9,8-opqraperylene-7,14-dione
Research Areas
Cancer, Apoptosis, Cancer Growth Inhibitors, Tyrosine Kinase Inhibitors
PubChem ID
5281051
Scientific Background
Hypericin is a naphtodianthrone that acts as an antibiotic and non-specific kinase inhibitor. It inhibits the action of dopamine beta-hydroxylase, leading to increased dopamine levels. Hypericin can also cause photosensitivity when ingested beyond threshold. And becase it also accumulates in cancerous tissues, it can be used as an indicator of cancerous cells (1, 2).
References
1. Hopfner M., et al. (2003) Int J Colorectal Dis. 18(3): 239-247.
2. Agostinis, et al. (2002) Int J Biochem Cell Biol. 34: 221.
Protein kinase C inhibitor
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
2. Agostinis, et al. (2002) Int J Biochem Cell Biol. 34: 221.
Hydroxychloroquine
Hydroxychloroquine__Autophagy inhibitor Palbociclib (isethionate)
Product Name
Hydroxychloroquine
Description
Autophagy inhibitor
Purity
>98%
CAS No.
747-36-4
Molecular Formula
C18H26ClN3O•H2SO4
Molecular Weight
433.95
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in greater than 20 mg/ml H2O.
Source
Synthetic
Appearance
White to off-white powder.
SMILES
C1=C2C(=CC(=C1)Cl)N=CC=C2NC(CCCN(CCO)CC)C.O=S(O)(O)=O
InChI
InChI=1S/C18H26ClN3O.H2O4S/c1-3-22(11-12-23)10-4-5-14(2)21-17-8-9-20-18-13-15(19)6-7-16(17)18;1-5(2,3)4/h6-9,13-14,23H,3-5,10-12H2,1-2H3,(H,20,21);(H2,1,2,3,4)
InChIKey
JCBIVZZPXRZKTI-UHFFFAOYSA-N
Safety Phrases
Classification: Acute toxicity, Oral (Category 4), Eye irritation (Category 2A)
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H302- Harmful if swallowed.
H319- Causes serious eye irritation.
Precautionary statements:
P264- Wash skin thoroughly after handling.
P270- Do not eat, drink or smoke when using this product.
P280- Wear protective gloves/ eye protection/ face protection.
P301 + P312- IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P305 + P351 + P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P330- Rinse mouth.
P337 + P313- If eye irritation persists: Get medical advice/ attention.
P501- Dispose of contents/ container to an approved waste disposal plant.
Cite This Product
Hydroxychloroquine (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-400)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132539
Alternative Names
Hydroxychloroquine sulphate, 7-Chloro-4-4-(N-ethyl-N-b-hydroxyethylamino)-1-methylbutylaminoquinoline
Research Areas
Cancer, Autophagy
PubChem ID
12947
Scientific Background
Hydroxychloroquine was first established as an anti-malarial agent, but is now being used in a variety of applications, including the inhibition of autopagy. Hydroxychloroquine is a lysosomotropic agent and therefore inhibits autophagy by obstructing the final step of autophagy, the fusion of autophagosomes with lysosomes, thereby preventing the acidification of the lysosomal compartment. In the context of cancer, autophagy is thought to be adopted by tumor cells to survive in the nutrient-deficient and hypoxic tumor environment. Hydroxychloroquine has therefore been studied extensively within the context of certain cancers.
References
1. van Loosdregt J., et al. (2013) J Allergy Clin Immunol. 131(5): 1443-6.e1.
2. Lotze M.T., et al. (2013) Cancer J. 19(4): 341-7.
3. Xie X., et al. (2013) PLoS One. 8(1): e55096.
Autophagy inhibitor
Safety Phrases:
S22 – Do not breathe dust.
S24/25 – Avoid contact with skin and eyes.
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection.
Hazard statements:
H302- Harmful if swallowed.
H319- Causes serious eye irritation.
Precautionary statements:
P264- Wash skin thoroughly after handling.
P270- Do not eat, drink or smoke when using this product.
P280- Wear protective gloves/ eye protection/ face protection.
P301 + P312- IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P305 + P351 + P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P330- Rinse mouth.
P337 + P313- If eye irritation persists: Get medical advice/ attention.
P501- Dispose of contents/ container to an approved waste disposal plant.
2. Lotze M.T., et al. (2013) Cancer J. 19(4): 341-7.
3. Xie X., et al. (2013) PLoS One. 8(1): e55096.
Huperzine A
Huperzine A__ACH inhibitor AZD-9292
Product Name
Huperzine A
Description
ACH inhibitor
Purity
>95% (HPLC); NMR (conforms)
CAS No.
102518-79-6
Molecular Formula
C15H18N2O
Molecular Weight
242.32
Storage Temperature
-20ºC
Shipping Temperature
Shipped Ambient
Product Type
Inhibitor
Solubility
Soluble in DMSO, methanol or ethanol.
Source
Synthetic
Appearance
Off-White solid
SMILES
C/C=C/1C@@H2CC3=C(C@1(CC(=C2)C)N)C=CC(=O)N3
InChI
InChI=1S/C15H18N2O/c1-3-11-10-6-9(2)8-15(11,16)12-4-5-14(18)17-13(12)7-10/h3-6,10H,7-8,16H2,1-2H3,(H,17,18)/b11-3+/t10?,15-/m1/s1
InChIKey
ZRJBHWIHUMBLCN-JYXJEATCSA-N
Safety Phrases
Classification: Toxic
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases:
H301+ H311 +H331 – Toxic if swallowed, in contact with skin or inhaled
H315 – Causes skin irritation.
H319 – Causes serious eye irritation.
H335 – May cause respiratory irritation.
Precautionary Phrases:
P261 – Avoid breathing dust/fume/gas/mist/vapours/spray.
P280 – Wear protective gloves/protective clothing.
P301+ P310 – IF SWALLOWED: Immediately call POISON CENTER or doctor/physician.
P305+ P351+ P338 – IF IN EYES: rinse cautiously with water for several minutes. If wearing contact lenses remove them and continue to rinse.
P311 – call a POISON CENTER or doctor/physician
Cite This Product
Huperzine A (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-365)
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132522
Alternative Names
(1R,9S,13E)- 1-Amino- 13-ethylidene- 11-methyl- 6-azatricyclo7.3.1.02,7 trideca- 2(7),3,10- trien- 5-one
Research Areas
Glutamate Receptors, Ion Channels, Neuroscience, Neurotransmitter Receptors, NMDA Receptors
PubChem ID
44461111
Scientific Background
Huperzine A is an acetylcholinesterase inhibitor. It is known for enhancing learning and memory by protecting a neurotransmitter molecule in the brain. Huperzine is also a NMDA glutamate receptor antagonist (1-2). The effects on the treatment of Alzheimer’s disease is under studies (1-3). Recent studies of Huperzine A highlight the potential therapeutic use for dementia by slowing down the neuronal cell death caused by glutamate (4).
References
1. Bai Dl., Tang Xc., He YC., Curr MedChem. (2000) 7(3): 355-74.
2. Tang L., Wang r., Tang x., (2005) Acta Acta Pharmacologica 26(6): 673-678.
3. Ved Hs,. Koenig ml., Dave JR., Doctor BP.(1997) Neurorport. 8(4): 963-8.
4. Li WM., Kan KK., Carlier PR., Pang YP., Han YF. YF., (2007) Curr Alzheimer Res. 4: 386-396.
ACH inhibitor
Safety Phrases:
S22 – Do not breathe dust
S24/25 – Avoid contact with skin and eyes
S36/37/39 – Wear suitable protective clothing, gloves and eye/face protection
Hazard Phrases:
H301+ H311 +H331 – Toxic if swallowed, in contact with skin or inhaled
H315 – Causes skin irritation.
H319 – Causes serious eye irritation.
H335 – May cause respiratory irritation.
Precautionary Phrases:
P261 – Avoid breathing dust/fume/gas/mist/vapours/spray.
P280 – Wear protective gloves/protective clothing.
P301+ P310 – IF SWALLOWED: Immediately call POISON CENTER or doctor/physician.
P305+ P351+ P338 – IF IN EYES: rinse cautiously with water for several minutes. If wearing contact lenses remove them and continue to rinse.
P311 – call a POISON CENTER or doctor/physician
2. Tang L., Wang r., Tang x., (2005) Acta Acta Pharmacologica 26(6): 673-678.
3. Ved Hs,. Koenig ml., Dave JR., Doctor BP.(1997) Neurorport. 8(4): 963-8.
4. Li WM., Kan KK., Carlier PR., Pang YP., Han YF. YF., (2007) Curr Alzheimer Res. 4: 386-396.
Anti-HSPB2 (MKBP) Antibody
Anti-HSPB2 (MKBP) Antibody__Rabbit Anti-Human HSPB2 (MKBP) Polyclonal MK-1776
Product Name
HSPB2 (MKBP) Antibody
Description
Rabbit Anti-Human HSPB2 (MKBP) Polyclonal
Species Reactivity
Human, Mouse, Rat
Applications
,
WB
,
ICC/IF
Antibody Dilution
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
Synthetic peptide
Concentration
1 mg/ml
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:
- High quantum yield
- Large phycobiliprotein
- 6 chromophores per molecule
- Isolated from red algae
- Molecular Weight: 105 kDa
Optical Properties:
λex = 650 nm
λem = 660 nm
εmax = 7.0×105
Φf = 0.68
Brightness = 476
Laser = 594 or 633 nm
Filter set = Cy®5
ATTO 390
Overview:
- High fluorescence yield
- Large Stokes-shift (89 nm)
- Good photostability
- Moderately hydrophilic
- Good solubility in polar solvents
- Coumarin derivate, uncharged
- Low molar mass: 343.42 g/mol
ATTO 390 Datasheet
Optical Properties:
λex = 390 nm
λem = 479 nm
εmax = 2.4×104
Φf = 0.90
τfl = 5.0 ns
Brightness = 21.6
Laser = 365 or 405 nm
ATTO 488
Overview:
Optical Properties:
λex = 501 nm
λem = 523 nm
εmax = 9.0×104
Φf = 0.80
τfl = 4.1 ns
Brightness = 72
Laser = 488 nm
Filter set = FITC
ATTO 565
Overview:
Optical Properties:
λex = 563 nm
λem = 592 nm
εmax = 1.2×105
Φf = 0.9
τfl = 3.4 n
Brightness = 10
Laser = 532 nm
Filter set = TRITC
ATTO 594
Overview:
Optical Properties:
λex = 601 nm
λem = 627 nm
εmax = 1.2×105
Φf = 0.85
τfl = 3.5 ns
Brightness = 102
Laser = 594 nm
Filter set = Texas Red®
ATTO 633
Overview:
Optical Properties:
λex = 629 nm
λem = 657 nm
εmax = 1.3×105
Φf = 0.64
τfl = 3.2 ns
Brightness = 83.2
Laser = 633 nm
Filter set = Cy®5
ATTO 655
Overview:
Optical Properties:
λex = 663 nm
λem = 684 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.8 ns
Brightness = 37.5
Laser = 633 – 647 nm
Filter set = Cy®5
ATTO 680
Overview:
Optical Properties:
λex = 680 nm
λem = 700 nm
εmax = 1.25×105
Φf = 0.30
τfl = 1.7 ns
Brightness = 37.5
Laser = 633 – 676 nm
Filter set = Cy®5.5
ATTO 700
Overview:
- High fluorescence yield
- Excellent thermal and photostability
- Quenched by electron donors
- Very hydrophilic
- Good solubility in polar solvents
- Zwitterionic dye
- Molar Mass: 575 g/mol
Optical Properties:
λex = 700 nm
λem = 719 nm
εmax = 1.25×105
Φf = 0.25
τfl = 1.6 ns
Brightness = 31.3
Laser = 676 nm
Filter set = Cy®5.5
FITC (Fluorescein)
Overview:
Optical Properties:
λex = 494 nm
λem = 520 nm
εmax = 7.3×104
Φf = 0.92
τfl = 5.0 ns
Brightness = 67.2
Laser = 488 nm
Filter set = FITC
PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:
Optical Properties:
λex = 535 nm
λem = 627 nm
Laser = 488 to 561 nm
PerCP
Overview:
Optical Properties:
λex = 482 nm
λem = 677 nm
εmax = 1.96 x 106
Laser = 488 nm
R-PE (R-Phycoerythrin)
Overview:
Optical Properties:
λex = 565 nm
λem = 575 nm
εmax = 2.0×106
Φf = 0.84
Brightness = 1.68 x 103
Laser = 488 to 561 nm
Filter set = TRITC
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Storage Buffer
PBS, 50% glycerol, 0.02% sodium azide
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Protein A purified
Clonality
Polyclonal
Specificity
Detects ~27kDa.
Cite This Product
Rabbit Anti-Human HSPB2 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-194)
Certificate of Analysis
1 µg/ml of SPC-194 was sufficient for detection of MKBP in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19132413
Alternative Names
DMPK-binding protein Antibody, heat shock 27kDa protein 2 Antibody, HSP27 Antibody, HSPB2 Antibody, MKBP Antibody, MGC133245 Antibody
Research Areas
Cancer, Heat Shock, Cell Signaling
Cellular Localization
Cytoplasm, Nucleus
Accession Number
NP_001532.1
Gene ID
3316
Swiss Prot
Q16082
Scientific Background
HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSPs, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP’s localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).
References
1. Pinz I., et al. (2008) FASEB J. 22: 84-92.
2. Suzuki A., et al. (1998) J Cell Bio. 140(5): 1113-1124.
3. Sugiyama Y., et al. (2000) J Biol Chem. 275: 1095-1104.
4. Oshita S.E., et al. (2010) Breast Cancer Res Treat. Epub: 20087649.
Rabbit Anti-Human HSPB2 (MKBP) Polyclonal
WB
,
ICC/IF
| APC (Allophycocyanin) | ||
Overview:
|
|
Optical Properties:
λex = 650 nm λem = 660 nm εmax = 7.0×105 Φf = 0.68 Brightness = 476 Laser = 594 or 633 nm Filter set = Cy®5 |
| ATTO 390 | ||
Overview:
ATTO 390 Datasheet |
|
Optical Properties:
λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
| ATTO 488 | ||
| Overview: | |
Optical Properties:
λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
| ATTO 565 | ||
| Overview: | |
Optical Properties:
λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
| ATTO 594 | ||
| Overview: | |
Optical Properties:
λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
| ATTO 633 | ||
| Overview: | |
Optical Properties:
λex = 629 nm λem = 657 nm εmax = 1.3×105 Φf = 0.64 τfl = 3.2 ns Brightness = 83.2 Laser = 633 nm Filter set = Cy®5 |
| ATTO 655 | ||
| Overview: | |
Optical Properties:
λex = 663 nm λem = 684 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.8 ns Brightness = 37.5 Laser = 633 – 647 nm Filter set = Cy®5 |
| ATTO 680 | ||
| Overview: | |
Optical Properties:
λex = 680 nm λem = 700 nm εmax = 1.25×105 Φf = 0.30 τfl = 1.7 ns Brightness = 37.5 Laser = 633 – 676 nm Filter set = Cy®5.5 |
| ATTO 700 | ||
Overview:
|
|
Optical Properties:
λex = 700 nm λem = 719 nm εmax = 1.25×105 Φf = 0.25 τfl = 1.6 ns Brightness = 31.3 Laser = 676 nm Filter set = Cy®5.5 |
| FITC (Fluorescein) | ||
| Overview: | |
Optical Properties:
λex = 494 nm λem = 520 nm εmax = 7.3×104 Φf = 0.92 τfl = 5.0 ns Brightness = 67.2 Laser = 488 nm Filter set = FITC |
| PE/ATTO 594 | ||
| PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. | ||
| Overview: | |
Optical Properties:
λex = 535 nm λem = 627 nm Laser = 488 to 561 nm |
| PerCP | ||
| Overview: | |
Optical Properties:
λex = 482 nm λem = 677 nm εmax = 1.96 x 106 Laser = 488 nm |
| R-PE (R-Phycoerythrin) | ||
| Overview: | |
Optical Properties:
λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
HRP (Horseradish peroxidase)
Properties:
- Enzymatic activity is used to amplify weak signals and increase visibility of a target
- Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
- High turnover rate enables rapid generation of a strong signal
- 44 kDa glycoprotein
- Extinction coefficient: 100 (403 nm)
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin
Properties:
Streptavidin
Properties:
- Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
- Molecular weight: 53 kDa
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
2. Suzuki A., et al. (1998) J Cell Bio. 140(5): 1113-1124.
3. Sugiyama Y., et al. (2000) J Biol Chem. 275: 1095-1104.
4. Oshita S.E., et al. (2010) Breast Cancer Res Treat. Epub: 20087649.