Cysteine carbamidomethylation was searched as a fixed modification, whilst lysine acetylation, serine/threonine/tyrosine phosphorylation, protein N-acetylation, and oxidized methionine have been searched as variable modifications. A greatest of two mis-cleavages was permitted even though we 834153-87-6 necessary rigorous tryptic specificity. The resulting Mascot.dat files ended up loaded into the MaxQuant software program jointly with the uncooked …
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