Ich is attributed to miR-206-mediated HDAC6 inhibition. Our investigation suggests that the usage of USCs-Exo represents a novel therapeutic strategy for stroke recovery. Funding: This perform was funded by National Natural Science Foundation of China (Numbers 81671209, 81471243 and 81472152).PF11.Protective effect of extracellular vesicles released in the neural stem cells on 6-hydroxydopamine induced pathological situation of Parkinson’s disease Eun Ji Leea, Dowon Hwangb and Dong Soo Leea Division of Nuclear Medicine, Seoul National University Hospital, Seoul, Republic of Korea; bSeoul National University Hospital, Seoul, Republic of Koreaapotential therapeutic sources for PD, NSCsecreted extracellular vesicles (EVs) like exosomes are key mediators of constructive paracrine effects. Direct proof for neuronal protective effects of EVs is crucial for building new PD therapeutics. Methods: To trace EV movement, a lentivirus containing Palm-tandem dimer tdTomato (Palm-td) was transduced into F3 NSCs. EVs isolated from Palm-tdinfected F3 cells showed high tdTomato fluorescence intensity sufficient to visualize their functional actions such as secretion, migration and engulfment among cells. We found that pretreatment with EVs drastically prevented 6-OHDA-induced toxicity by minimizing intracellular reactive oxygen species (ROS), percentage of apoptotic cells and caspase-3/7 activity. Results: These benefits indicate that NSC-derived EVs have neuroprotective effects against the cell harm, possibly by means of antioxidant and anti-apoptotic action. Specifically, F3-derived EVs correctly prevents 6OHDA-induced the production of ROS, NSC-EVs that inhibit ROS production may well be applied as an important therapeutic agent for neuroprotection. EVmediated neuroprotection likely acts by inhibiting the generation of caspase-3/7, top to minimize apoptosis induction brought on by 6-OHDA neurotoxicity. Summary/Conclusion: In summary, this study demonstrates that NSC-derived EVs protected dopaminergic cells from T-type calcium channel site oxidative insults. Though 6-OHDA-induced cell death in SH-SY5Y cells happens by the generation of ROS, the neurotoxin-mediated cell death was suppressed by F3-derived EVs by way of their protective effects on ROS-induced cell damage. We for that SMYD2 Storage & Stability reason count on that additional investigations into the therapeutic applications of NSC-derived EVs will reveal further advantages for EV-based PD therapies in comparison to cell transplantation.PF11.The function of extracellular vesicles secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells on a cellular ischemic stroke model Gang Lu, Man Sze Wong, Xian Wei Su, Rui Can Cao, Hoi Hung Cheung and Wai Yee Chan CUHK-SDU joint laboratory on reproductive genetics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, Hong KongIntroduction: Parkinson’s illness (PD) is often a neurodegenerative illness characterized by bradykinesia, resting tremors and postural instability. A key symptom of PD is the loss of the nigral dopaminergic neurons and subsequent dopamine deficit in the brain. However, the precise mechanism continues to be unknown. When neural stem cells (NSCs) areIntroduction: Stroke is definitely the second top reason for death along with the key cause of long-term disability, but yet lack of helpful therapy. Research recommended the transplantation of mesenchymal stem cells (MSCs) improved recovery from stroke in animalJOURNAL OF EXTRACELLULAR VESICLESmodels, similar therapeutic impact was fou.