Re made use of as a optimistic handle. Right after five days of incubation at 37 in an atmosphere of 5 CO2, [3H]thymidine (0.5 Ci/well) was added to the cultures, and incorporated radioactivity was measured by scintillation counting 16 h later. The outcomes had been expressed as imply counts per minute (cpm) from triplicate wells. …
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