Inoma PANC-1 and BxPC3 cell lines were purchased from the Cell Bank on the Chinese Academy of Science (Shanghai, China) and cultured in DMEM (Corning, Beijing, China) supplemented with ten FBS (Sigma, Shanghai, China) and 1 Penicillin treptomycin (Sigma, Shanghai, China) at 37 C with five CO2 . Within the exponential phase of the growth, cells were plated onto 96-well 3-Chloro-5-hydroxybenzoic acid MedChemExpress plates at a concentration of 8000 cells/well for 24 h. Compounds 1 have been ready to a variety of concentrations (80, 40, 20, ten, five, two.five, 1.25, and 0.625 in medium containing less than 0.1 DMSO) and incubated in 96-well plates (each and every concentration in six-fold wells) for 72 h. Gemcitabine (Gem, Meilunbio, 98 , Dalian, China) was presented because the good handle. Cell viability was determined based on reported assay techniques working with the commercial CCK8 kit (Elabscience, Wuhan, China) [29]. The optical density (OD) of each and every nicely was measured with an AMR-100 microplate reader at 450 nm (Allsheng Corporation, Hangzhou, China). Cytotoxicity emerged because the value on the drug concentration in the inhibition of cell development by 50 (IC50 ). four. Conclusions This study afforded ten compounds from the rhizomes of Paris mairei, like five new spirostane saponins. None of the new compounds exhibited cytotoxicity against PANC-1 and BxPC3 pancreatic cell lines, implying that the polyglucosides at 1-hydroxy in spirostane saponins may significantly decreased the activities of antitumor.Supplementary Supplies: The following are out there on-line, Figures S1 55: NMR (1D and 2D) and MS spectra of compounds 1. Author Contributions: Conceptualization, H.T. and B.-L.Z.; NMR data, M.W.; acid hydrolysis and GC analysis, P.Q.; cytotoxicity evaluation, H.H.; isolation, structural identification, and writing– original draft preparation, Y.L. (Yang Liu); writing–review and editing, Y.L. (Yunyang Lu). All authors have study and agreed towards the published version of the manuscript. Funding: The research operate was financially supported by the National Natural Science Foundation of China (No. 81973192 and No.81903862) and also the Shaanxi Province Important Research and Improvement Projects of China (No.2021ZDLSF04-07 and No. 2020SF-311). Institutional Review Board Statement: Not applicable. Informed Consent Statement: Not applicable. Data Availability Statement: The information used to assistance the findings of this study are readily available in the corresponding author upon request. Conflicts of Interest: The authors declare no conflict of interest. Sample Availability: Samples in the compounds 60 are offered in the authors.Molecules 2021, 26,15 of
moleculesArticleInfluence of Supercritical Carbon Dioxide Extraction Situations on Extraction Yield and Composition of Nigella sativa L. Seed Oil–Modelling, Optimization and Extraction Kinetics regarding Fatty Acid and Thymoquinone ContentGrzegorz Gawron 1 , Wojciech Krzyczkowski 2 , Robert Ly en 1 , Leszek Kadzinski 1, z and Bogdan BaneckiIntercollegiate Faculty of Biotechnology of University of Gdansk and FAUC 365 Protocol Health-related University of Gdansk, University of Gdansk, Abrahama Str. 58, 80-307 Gdansk, Poland; [email protected] (G.G.); [email protected] (R.L.); [email protected] (B.B.) Biovico Sp. z o.o., Hutnicza Str. 15 B, 81-061 Gdynia, Poland; [email protected] Correspondence: [email protected]: Gawron, G.; Krzyczkowski, W.; Lyzen, R.; Kadzinski, L.; Banecki, B. Influence of Supercritical Carbon Dioxide Extraction Co.