A, Ottawa, Canada; bAmsterdam UMC, University of Amsterdam, Division of Biomedical Engineering and Physics, Amsterdam, NetherlandsIntroduction: Many research have shown that plantderived nanoparticles (NPs) taken up by the intestinal cells influence intestinal function. Food-derived NP is recognized to facilitate delivery of proteins, nucleic acids like microRNA (miRNA) as well as other significant molecules to intestinal tissues. Consequently, such substantial molecules might impact gastrointestinal functions via NPs. Accordingly, we investigated the impact of applederived NP to intestinal transporters via containing cargos. Procedures: NP was prepared by ultracentrifugation. Lipid membrane of NP and apple-derived nucleic acid have been labelled by fluorescents to examine uptake in Caco-2 cells working with microscope. Expressions of mRNA and protein of transporters in Caco-2 cellsIntroduction: Nanoscale flow cytometry (NFC) is often a promising tool for phenotypic evaluation of individual modest particles for instance extracellular vesicles (EVs) and viruses which might be smaller sized than 500 nm in diameter. Nonetheless, because lots of compact EVs are at present in the limit of detection for commercial flow cytometers, successful detection of EVs calls for optimization of each sample preparation and instrument settings. These optimizations need reference particles reflecting size, refractive index (RI), and fluorescence emission intensity in the labelled EVs of interest. Murine leukaemia virus (MLV) is actually a retrovirus 114 nm in diameter as measured by cryo-EM, with an estimated RI of 1.five. Here we showcase the monodispersed nature of these viruses and demonstrate their use as fluorescence reference particles for NFC.ISEV2019 ABSTRACT BOOKMethods: We engineered MLVs to express its envelope glycoprotein fused to green fluorescent proteins (eGFP and sfGFP) around the viral surface. MLVs have been characterized by NFC and by nanoparticle tracking analysis. Since MLVs are monodispersed, we combined scatter intensities and hydrodynamic diameter to get the successful RI by solving the inverse light scattering dilemma making use of Mie theory. Benefits: We measured an antigen CD85d/ILT-4 Proteins Storage & Stability density of 300 MESF of GFP per virion. BTN2A1 Proteins supplier Additionally, we located that antibody labelling of this virus-associated antigen with distinctive fluorophore conjugates (PE, BV421 and AF647) modulates each scatter intensities and hydrodynamic diameter in the labelled virus. With regard for the hydrodynamic diameter, we show that the effectiveRI on the viruses could possibly be tuned by utilizing distinctive fluorophores. Summary/Conclusion: MLVs are equivalent to modest EVs in size with equivalent surface area and comparable capacity for antigen expression. Unlike synthetic beads, MLVs is often genetically engineered to express protein antigens of decision in biologically relevant and consistent levels to act as internal constructive controls for phenotypic studies of EV surface marker expression. In addition, MLVs are monodisperse and have tuneable RI. Collectively, these properties support that MLVs are robust candidates as fluorescence reference particles for NFC. Funding: Organic Sciences and Engineering Analysis Council of Canada (NSERC)JOURNAL OF EXTRACELLULAR VESICLESPF07: Biogenesis II Chairs: Mathilde Mathieu; Hang Hubert Yin Place: Level 3, Hall A 15:306:PF07.Proteomic profiling of outer membrane vesicles derived from MicA, a smaller RNA from Escherichia coli So Hee Leea, Yeong-Jun Parkb and Kwang-sun Kima Pusan National University, Busan, Republic of Korea; bPusan National Unive.