Odies in relevant in vitro models. Coupling immune-isolation to pre-analytical protocols for urine processing and sample excellent testing enables detection of a panel of endometrial genes in urine-recovered EVs. Summary/Conclusion: General, the study supplies a tool for non-invasive monitoring with the functional status with the endometrium, supporting biomedical niches which include assisted fertilization and diagnosis of endometriosis. Funding: ENDEvor POR Region Tuscany (identification on the project) and Exosomics R D ProgrammePF09.Unveiling autologous blood doping: comparative evaluation of unique purification tactics for urinary extracellular vesicles pioneering miRNA biomarker study Veronika Mussacka, Georg Wittmannb and Michael Pfafflc TUM School of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany, Freising, Germany; bDepartment for Transfusion Medicine, Cell therapeutics and Haemostaseology, University Hospital LMU, Munich, Germany, M chen, Germany; cAnimal Physiology and Immunology, College of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany, Freising, Germanyafive distinctive isolation techniques had been evaluated: ultracentrifugation, membrane affinity, spin column chromatography, 5-HT1 Receptor Inhibitor supplier immunoaffinity and precipitation. After EV characterization by nanoparticle tracking analysis, western blotting, and transmission electron microscopy, total RNA was isolated in addition to a library for modest RNA sequencing was prepared. The resultant profitable method was then applied to all of the collected samples which were equally analysed concerning their EV distribution and miRNA content material. Benefits: The comparative analysis disclosed large discrepancies with respect to EV yield, population, and purity, too as RNA yield and detected miRNAs. By applying the most effective performing method, which was according to immunoaffinity, drastically greater amounts of urinary EVs and several drastically differentially regulated miRNAs had been observed right after ABD. Summary/Conclusion: Urinary EVs and their miRNA profile hold certainly promising attempts for the clear separation of ABD and non-doped athletes. In addition, the integrated complicated comparative methodological evaluation contributes enormously to future standardization and comparability of urinary EV analysis. Funding: The present project has been financially supported by the WADA.PF09.Extracellular vesicles as graft biomarkers to address lung transplantation outcome Mario Barilania, Ilaria Righib, Giuseppe Buonoc, Lorenzo Rossod, Mario Nosottie and Lorenza Lazzarica Unit of Regenerative Medicine Cell Factory, Department of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy; Universitdegli Studi di Milano, Milan, Italy; bThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; cUnit of Regenerative Medicine Cell Factory, Department of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; dUniversitdegli Studi di Milano, Milano (MI), Italy, Milano, ItalyIntroduction: Autologous blood doping (ABD) increases the P/Q-type calcium channel manufacturer oxygen capacity by means of re-infusion of a person’s autologous red blood cells. It is actually consequently applied by endurance athletes with a higher level of unreported circumstances, specially considering that trustworthy strategies for unequivocal detection are nevertheless lacking. To su.