Enitor neurons) and collagen IV (angiogenesis) of ipsilateral. EVs groups showed significantly improved co-expression of ki-67 and DCX within the subventricular zone. Enhanced expression of collagen IV within the ischemic boarder zone was much more identified within the EV groups than in the manage group. Diffusion tensor imaging was made use of to examine the regeneration of nerve fibre bundles, and nerve fibre bundles were also enhanced in the EV groups. Summary/Conclusion: Our study shows that WJ-MSC derived EVs from 3D culture system promotes the neurogenesis, angiogenesis and recovery of nerve fibre bundles in rat 4-1BB Inhibitor medchemexpress stroke models. These final results recommend that an efficient MSC-derived EV can be employed ideally for the treatment of stroke. Funding: This study was supported by a grant in the Korean Healthcare Technology R D Project, Ministry of Well being Welfare (HI17C1256) and Standard Science Research Program, the Ministry of Science, ICT and Future Arranging (2018M3A9H1023675).PF11.Exosomes from human urine-derived stem cells market neurogenesis through histone deacetylase6 regulation in ischemic stroke Xiaozheng Linga, Zhang Guoweia, Qingwei Zhua, Hu Guowena, Yang Wangb and Zhifeng Dengaa Shanghai Jiao Tong University Affliated Sixth People’s Hospital, Shanghai, China (People’s Republic); bShanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China (People’s Republic)PF11.The therapeutic prospective of MSC-derived extracellular vesicles for stroke Ji Hee Sunga, Eun Kyoung Shinb, Jeong Pyo Sonc, Yeon Hee Choa, Dong Hee Kimd, Mi Jeong Ohb, Eun Hee Kimb, Jae Min Chae, Oh Young Bangfa Samsung health-related center, seoul, Republic of Korea; bSamsung health-related center, Seoul, Republic of Korea; cSungkyunkwan University, Seoul, Republic of Korea; d Sungkyunkwan University, seoul, Republic of Korea; eDepartment of Mechatronics, College of Engineering, Incheon National University, Incheon, Republic of Korea; fSamsung healthcare center, Seoul, Republic of KoreaIntroduction: Human urine-derived stem cells (USCs) are receiving significantly more interest in tissue regeneration and injury repair. Exosomes derived from USCsISEV2019 ABSTRACT BOOK(USCs-Exo) have recently been recommended to mediate the restorative effects of USCs. On the other hand, no matter if USCs-Exo play a protective function in stroke remains unknown. Methods: Therapeutic effect of USCs-Exo in vivo was evaluated by cerebral Adenosine A3 receptor (A3R) Agonist review infarction and neurological behaviour. The proliferation and differentiation of neural stem cells (NSCs) was determined by double immunofluorescent staining. The viability, apoptosis, proliferation, and differentiation of NSCs subjected to oxygen glucose deprivation/reoxygenation (OGD/R) were assessed, respectively. The protein expression and activity of HDAC6, plus the expression levels of miRNAs each in vivo and in vitro had been assessed to detect the probable mechanism. Final results: We identified that intravenous injection of USCsExo lowered brain infarct volume and improved functional recovery by enhancing the proliferation and differentiation of NSCs in ischemic rats. The in vitro final results recommended that USCs-Exo boost viability, decrease apoptosis, and market the proliferation and neuronal differentiation of NSCs following OGD/R. We further identified that miR-206 contained in USCs-Exo is related with all the therapeutic efficacy for neurogenesis via the inhibition of histone deacetylase6 (HDAC6). Summary/Conclusion: Our study demonstrates that USCs-Exo can boost neurological function recovery by promoting neurogenesis, wh.