Strain causes birth defects in experimental modelsFernanda R. Cugola1,sirtuininhibitor Isabella R. Fernandes1,2,sirtuininhibitor Fabiele B. Russo1,3,sirtuininhibitor Beatriz C. Freitas2, Jo L.M. Dias1, Katia P. Guimar s1, Cec ia Benazzato1, Nathalia Almeida1, Graciela C. Pignatari1,3, Sarah Romero2, Carolina M. Polonio4, Isabela Cunha4, Carla L. Freitas4, Wesley N. Brand four, Cristiano Rossato4, David G. Andrade4, Daniele de P. Faria5, Alexandre T. Garcez5, Carlos A.. Buchpigel5, Carla T. Braconi6, Erica Mendes6, Amadou A. Sall7, Paolo M. de A. Zanotto6, Jean Pierre S. Peron4,, Alysson R. Muotri2,, and Patricia C. B. Beltr -Braga1,eight,1Universityof S Paulo, Division of Surgery, Stem Cell Laboratory, S Paulo, S Paulo, 05508-270, Brazil2Universityof California San Diego, School of Medicine, Division of Pediatrics/Rady Children’s Hospital San Diego, Division of Cellular Molecular Medicine, Stem Cell System, La Jolla, CA 92037-0695, USA3Tismoo,The Biotech Company, S Paulo, SP, 01401-000, Brazil4Universityof S Paulo, Division of Immunology, Neuroimmune Interactions Laboratory, S Paulo, SP, 05508-000, Brazil5Universityof S Paulo, Division of Radiology and Oncology, USP College of Medicine, S Paulo, SP, 05403-010, BrazilUsers could view, print, copy, and download text and data-mine the content material in such documents, for the purposes of academic research, subject normally towards the complete Conditions of use: nature/authors/editorial_policies/license.html#termsReprints and permission facts is available at www.nature/reprints. To whom correspondence should be addressed: Dr. Beltr -Braga, Av, Prof. Dr. Orlando Marques de Paiva, 87. Cidade Universit ia. CEP: 05508-270. S Paulo, SP. Brazil. [email protected]. Telephone: +55 (11) 3091-1312. Dr. Muotri, 2880 Torrey Pines Scenic Drive, La Jolla, CA 92037.BMP-7 Protein site MC0695, [email protected], Phone: +1 (858) 534-9320. Dr. Jean Pierre S. Peron, Av. Prof. Dr. Lineu Prestes, 1730, Cidade Universit ia. CEP: 05508-270. S Paulo, SP. Brazil. [email protected], Phone: +55 (11) 3091-7430. hese authors contributed equally to this function. Supplementary Information is linked to the on-line version in the paper at www.nature/nature. Author Contributions F.R.G. derived the NPCs, neurons and neurospheres, performed the viral infections and cell analyses and analyzed the information. I.R.F. performed the viral infections of cells, processed and analyzed infected brain organoids, prepared manuscript figures and analyzed the data. F.B.R. derived the NPCs, performed immunostainings and analyses, ready manuscript figures and analyzed the data. B.C.F. revised the manuscript and with S.R. generated the organoid cultures from iPSCs and assisted using the IF staining and experimental style. J.L.M.D. performed macroscopic and microscopic staining and analyses from the mice.MKK6 Protein Purity & Documentation K.PMID:25105126 O.P.G. performed the TEM experiments, RNA extraction and quantification and histopathological analyses. C.B. and N.S. performed RNA extraction and quantification and ready figures. G.C.P. performed cell culture, analyzed the data and revised the manuscript. C.M.P., I.C., C.L.F., W.N.B. and C.R. performed cell death q PCR array and flow cytometry, D.G.A performed flow cytometry staining protocols and analyzed the data. C.M.P, I.C. and D.G.A. infected and observed the pregnant mice each day. C.M.P., C.L.F., I.C. and C.R. processed the mouse tissues for virus quantification and further analyses. D.P.F., A.T.G. and C.A.B. performed the CT scans and analyzed and plot.